Science Journal of Chemistry

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Compound Identification by HPLC-ESI-Q-TOF-MS/ MS Analysis of the Dichloromethane Fraction of Hyptis suaveolens Leaves After Extraction of the Essential Oil

Received: 17 December 2023    Accepted: 5 January 2024    Published: 1 February 2024
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Abstract

Hyptis suaveolens (Lamiaceae), widespread in tropical areas of America, Asia, and Africa, is used in more than twenty-two countries for its medicinal properties. The plant's leaves contain polyphenols, tannins, sterols and terpenes, saponins, flavonoids, quinones and anthraquinones before and after extraction of the essential oil. However, no molecular structures were identified in the leaves of the plant after extraction of the essential oil. So, after hydroethanolic (70/30; v/v) maceration of 100g of powder front and after extracting essential oil taken separately, a successively fractionate extract was obtained from solvents of increasing polarities. Next, the compounds of the dichloromethane fraction after extracting essential oil have been identified by HPLC-ESI- Q-TOF -MS/ MS method. As a result, the determination of the structures of nine (9) known compounds of the Hyptis genus was carried out by dereplication on the dichloromethane fraction from the hydroalcoholic extract (70%) of the plant's leaves after extraction of the essential oil. Of these nine (09) compounds, four (04) are phenolics and the remaining five (05) are terpenoids. Five (05) of these molecules had not yet been identified in Hyptis suaveolens leaves. This study shows that Hyptis suaveolens leaves after extraction of the essential oil are new source of bioactive compounds of pharmacological interest.

DOI 10.11648/sjc.20241201.11
Published in Science Journal of Chemistry (Volume 12, Issue 1, February 2024)
Page(s) 1-14
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hyptis suaveolens, Dereplication, Molecular Structure, After Extraction of Essential Oil

References
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[2] M. J. Oliveira et al, "Influence of growth phase on the essential oil composition of Hyptis suaveolens," Biochem. Syst. Ecol. vol. 33(3), pp. 275-285, 2005.
[3] E. V Tia et al, "Chemical composition and insecticidal activity of essential oils of two aromatic plants from Ivory Coast against Bemisia tabaci G. (Hemiptera: Aleyrodidae)," Nat. Prod. Commun. vol. 6, no. 8, pp. 1183-8, Aug. 2011, Accessed: Mar. 05, 2019. [Online]. Available: http://www.ncbi.nlm.nih.gov/pubmed/21922931.
[4] S. Kumar, Nandha, and N. Thampi, "Phytochemical screening and characterization of the bioactive compounds from the leaves of Hyptis suaveolens and Spathodea campanulata," J. Chem. Pharm. Res. vol. 7, no. 7, pp. 840-850, 2015, Accessed: Mar. 05, 2019. [Online]. Available: www.jocpr.com.
[5] B. Soumahoro, Y. Soro, A. K. B. Brise, and S. Siaka, "Etude comparative des caractéristiques phytochimiques des feuilles de Hyptis suaveolens avant et après extraction de l ' huile essentielle, ” J. Soc. Ouest-Afr. Chim., vol. 049, pp. 1–8, 2020.
[6] K. R. C. Goly, Y. SORO, A. DADIE, and A. B. B. and D. M. KASSI, "Antibacterial activity of essential oils and extracts from the leaves of Hyptis suaveolens and Lippia multiflora on multi-resistant bacteria," rasayan j. Chem. vol. 8, no. 4, pp. 396-403, 2015, Accessed: Mar. 05, 2019. [Online]. Available: http://www.rasayanjournal.comhttp//www.rasayanjournal.co.in.
[7] B. Soumahoro, K. T. Konan, A. B. Benjamin, and Y. Soro, "Comparative study of Hyptis suaveolens leaves antioxidant activity before and after essential oil extraction," Int. J. Chem. Stud. vol. 11, no. 5, pp. 27-33, 2023, doi: 10.22271/chemi.2023.v11.i5a.12348.
[8] H. Bouamama, T. Noël, J. Villard, A. Benharref, and M. Jana, "Antimicrobial activities of the leaf extracts of two Moroccan Cistus L. species," J. Ethnopharmacol. vol. 104, pp. 104-107, Mar. 2006, doi: 10.1016/j.jep.2005.08.062.
[9] J. K. Jongmin A, Hee-Sung C, Young-Won C, "Dereplication-Guided Isolation of New Phenylpropanoid-, Substituted Diglycosides from Cistanche salsa and Their Inhibitory Activity on NO Production in Macrophage," Molecules, vol. 22, p. 1138, 2017.
[10] F. L. McFarland K, Mulholland DA, "Limonoids from Turraea floribunda (Meliaceae)," Phytochemistry, vol. 65, pp. 2031-2037, 2004.
[11] B. Soumahoro, K. P. F. O. Kone, G. Y. T. Douhore, and Y. Soro, “Identification of compounds by HPLC-ESI-Q-TOF-MS/MS analysis of the dichloromethane fraction from the hydroalcoholic extract of Hyptis suaveolens leaves before extraction of the essential oil,” J. Mater. Environ. Sci., vol. 14(12), 1529-1550, 2023.
[12] K. Mukherjee, R. Mukherjee, and P. Ghosh, "Chemistry of Hyptis suaveolens: a pentacyclic triterpene," J. Nat. Prod, vol. 47(2), pp. 377-8, 1984.
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[14] Joseph, Joselin, Jeeva, and Solomon, "GC-MS and FT-IR analysis of a coastal medicinal plant- Hyptis suaveolens (L.) Poit," J. Coast. Life Med. vol. 4, no. 5, pp. 380-385, 2016, doi: 10.12980/jclm.4.2016J5-147.
[15] G. A. Suárez-Ortiz, C. M. Cerda-García-Rojas, M. Fragoso-Serrano, and R. Pereda- Miranda, "Complementarity of DFT Calculations, NMR Anisotropy, and ECD for the Configurational Analysis of Brevipolides K-O from Hyptis brevipes," J. Nat. Prod. vol. 80, no. 1, pp. 181-189, Jan. 2017, doi: 10.1021/acs.jnatprod.6b00953.
[16] R. Malele, C. Mutayabarwa, and G. Mwangi, JW and Thoithi, "Essential Oil of Hyptis suaveolens (L.) Poit. from Tanzania: Composition and Antifungal Activity," J. Essent. Oil Res. vol. 15(6), pp. 438-40, 2003.
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  • APA Style

    Soumahoro, B., Bohui, G. S., Kalo, M., Kanaté, L., Attioua, B., et al. (2024). Compound Identification by HPLC-ESI-Q-TOF-MS/ MS Analysis of the Dichloromethane Fraction of Hyptis suaveolens Leaves After Extraction of the Essential Oil. Science Journal of Chemistry, 12(1), 1-14. https://doi.org/10.11648/sjc.20241201.11

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    ACS Style

    Soumahoro, B.; Bohui, G. S.; Kalo, M.; Kanaté, L.; Attioua, B., et al. Compound Identification by HPLC-ESI-Q-TOF-MS/ MS Analysis of the Dichloromethane Fraction of Hyptis suaveolens Leaves After Extraction of the Essential Oil. Sci. J. Chem. 2024, 12(1), 1-14. doi: 10.11648/sjc.20241201.11

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    AMA Style

    Soumahoro B, Bohui GS, Kalo M, Kanaté L, Attioua B, et al. Compound Identification by HPLC-ESI-Q-TOF-MS/ MS Analysis of the Dichloromethane Fraction of Hyptis suaveolens Leaves After Extraction of the Essential Oil. Sci J Chem. 2024;12(1):1-14. doi: 10.11648/sjc.20241201.11

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  • @article{10.11648/sjc.20241201.11,
      author = {Brahima Soumahoro and Gouegoui Serge-Pacôme Bohui and Mabintou Kalo and Losseyni Kanaté and Barthélémy Attioua and Yaya Soro},
      title = {Compound Identification by HPLC-ESI-Q-TOF-MS/ MS Analysis of the Dichloromethane Fraction of Hyptis suaveolens Leaves After Extraction of the Essential Oil},
      journal = {Science Journal of Chemistry},
      volume = {12},
      number = {1},
      pages = {1-14},
      doi = {10.11648/sjc.20241201.11},
      url = {https://doi.org/10.11648/sjc.20241201.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.sjc.20241201.11},
      abstract = {Hyptis suaveolens (Lamiaceae), widespread in tropical areas of America, Asia, and Africa, is used in more than twenty-two countries for its medicinal properties. The plant's leaves contain polyphenols, tannins, sterols and terpenes, saponins, flavonoids, quinones and anthraquinones before and after extraction of the essential oil. However, no molecular structures were identified in the leaves of the plant after extraction of the essential oil. So, after hydroethanolic (70/30; v/v) maceration of 100g of powder front and after extracting essential oil taken separately, a successively fractionate extract was obtained from solvents of increasing polarities. Next, the compounds of the dichloromethane fraction after extracting essential oil have been identified by HPLC-ESI- Q-TOF -MS/ MS method. As a result, the determination of the structures of nine (9) known compounds of the Hyptis genus was carried out by dereplication on the dichloromethane fraction from the hydroalcoholic extract (70%) of the plant's leaves after extraction of the essential oil. Of these nine (09) compounds, four (04) are phenolics and the remaining five (05) are terpenoids. Five (05) of these molecules had not yet been identified in Hyptis suaveolens leaves. This study shows that Hyptis suaveolens leaves after extraction of the essential oil are new source of bioactive compounds of pharmacological interest.
    },
     year = {2024}
    }
    

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  • TY  - JOUR
    T1  - Compound Identification by HPLC-ESI-Q-TOF-MS/ MS Analysis of the Dichloromethane Fraction of Hyptis suaveolens Leaves After Extraction of the Essential Oil
    AU  - Brahima Soumahoro
    AU  - Gouegoui Serge-Pacôme Bohui
    AU  - Mabintou Kalo
    AU  - Losseyni Kanaté
    AU  - Barthélémy Attioua
    AU  - Yaya Soro
    Y1  - 2024/02/01
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    DO  - 10.11648/sjc.20241201.11
    T2  - Science Journal of Chemistry
    JF  - Science Journal of Chemistry
    JO  - Science Journal of Chemistry
    SP  - 1
    EP  - 14
    PB  - Science Publishing Group
    SN  - 2330-099X
    UR  - https://doi.org/10.11648/sjc.20241201.11
    AB  - Hyptis suaveolens (Lamiaceae), widespread in tropical areas of America, Asia, and Africa, is used in more than twenty-two countries for its medicinal properties. The plant's leaves contain polyphenols, tannins, sterols and terpenes, saponins, flavonoids, quinones and anthraquinones before and after extraction of the essential oil. However, no molecular structures were identified in the leaves of the plant after extraction of the essential oil. So, after hydroethanolic (70/30; v/v) maceration of 100g of powder front and after extracting essential oil taken separately, a successively fractionate extract was obtained from solvents of increasing polarities. Next, the compounds of the dichloromethane fraction after extracting essential oil have been identified by HPLC-ESI- Q-TOF -MS/ MS method. As a result, the determination of the structures of nine (9) known compounds of the Hyptis genus was carried out by dereplication on the dichloromethane fraction from the hydroalcoholic extract (70%) of the plant's leaves after extraction of the essential oil. Of these nine (09) compounds, four (04) are phenolics and the remaining five (05) are terpenoids. Five (05) of these molecules had not yet been identified in Hyptis suaveolens leaves. This study shows that Hyptis suaveolens leaves after extraction of the essential oil are new source of bioactive compounds of pharmacological interest.
    
    VL  - 12
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Author Information
  • Department of Agro-Food Chemical Engineering, National Polytechnic Institute Félix Houphouët-Boigny, Yamoussoukro, Ivory Coast

  • Department of Physics Chemistry, Félix Houphouët-Boigny University, Abidjan, Ivory Coast

  • Department of Agro-Food Chemical Engineering, National Polytechnic Institute Félix Houphouët-Boigny, Yamoussoukro, Ivory Coast

  • Department of Agro-Food Chemical Engineering, National Polytechnic Institute Félix Houphouët-Boigny, Yamoussoukro, Ivory Coast

  • Department of Physics Chemistry, Félix Houphouët-Boigny University, Abidjan, Ivory Coast

  • Department of Agro-Food Chemical Engineering, National Polytechnic Institute Félix Houphouët-Boigny, Yamoussoukro, Ivory Coast

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