Science Journal of Chemistry

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Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae)

Received: 16 January 2024    Accepted: 30 January 2024    Published: 21 February 2024
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Abstract

The phytochemical investigation of a previously unstudied species of the genus Apocynaceae, Baissea mortehanii de Wild was undertaken and eight known secondary metabolites were isolated from leaves of this plant including one alkaloid, N-Feruloyltryptamine (1); one aromatic ester, Dibutyl phthalate (2); two flavonoids, Genistein (3) and Gerontoisoflavone A (4), four sterols, β-Sitosterol (5), Sitosterol-3-O-β-D-glucopyranoside (6), Stigmasterol (7) and Stigmasterol-3-O-β-D glucopyranoside (8). The structures of compounds were determined by means of spectroscopic methods :NMR analysis (1H and13C NMR, 1H-1H-COSY, HSQC, HMBC), spectrometric methods such as UV, IR, ESI-MS, EI, and by comparing their data with those reported in the literature. All the isolated compounds were tested for their potential to inhibit the enzyme urease. Urease activity was determined by measuring ammonia production using the indophenol method and thiourea was used as standard inhibitor of urease. Compounds 5 and 7 showed the best urease inhibition with an IC50 value 17. 2 and 18.5 µM respectively, which is higher than that of the potent inhibitor, thiourea (IC50 = 21.5 µM); Compounds 3, 4, 6 and 8 showed a good urease inhibition with an IC50 value 26.9, 29.7, 32.8 and 34.3 µM respectively; Compounds 1 and 2 showed a moderate urease inhibition with an IC50 value 49.1 and 46.8 µM respectively.

DOI 10.11648/j.sjc.20241201.13
Published in Science Journal of Chemistry (Volume 12, Issue 1, February 2024)
Page(s) 23-26
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Apocynaceae, Baissea Mortehanii, Enzyme Urease

References
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[5] Khan KM, Iqbal S, Lodhi MA, Maharvi GM, Zia-Ullah, Choudhary MI, Rahman A-U, Perveen S. 2004. Biscoumarin: new class of urease inhibitors; economical synthesis and activity. Bioorg Med Chem. 12(8): 1963–1968.
[6] Achika, J. I., Arthur, D. E., Gerald, I., Adedayo, A. (2014). A review on the phytoconstituents and related medicinal properties of plants in the Asteraceae family. IOSR J Appl Chem, 7(8), 1-8.
[7] Ruikar, AD, Gadkari, TV, Phalgune, UD, Puranik, VG et Deshpande, NR (2011). Phtalate de dibutyle, un métabolite secondaire de Mimusops elengi. Chimie des composés naturels, 46, 955-956.
[8] Yasuda, T., Mizunuma, S., Kano, Y., Saito, K. I., Ohsawa, K., 1996. Urinary and biliary metabolites of genistein in rats. Biol. Pharm. Bull. 19, 413–417.
[9] Likhitwitayawuid, K., Kaewamatawong, R., Ruangrungsi, N., 2005. Mono- and biflavonoids of Ochna integerrima. Biochem. Syst. Ecol., 33: 527.
[10] Welter, W., Bertina, M., Nuno, A. P., 2000. Natural plant products active against snakebite, the molecular approach. Phytochem. 55, 463–482.
[11] Mizushina, Y., Nakanishi, R., Kuriyama, I., Kamiya, K., Satake, T., Shimazaki, N., Koiwai, O., Uchiyama, Y., Yonezawa, Y., Takemura, M., Sakaguchi, K., Yoshida, H., 2006. β sitosterol-3-O-β-D-glucopyranoside: A eukaryotic DNA polymerase λ inhibitor. J. Steroid Biochem. 99, 100–107.
[12] Luhata, L. P., Munkombwe, N. M., 2015. Isolation and characterisation of Stigamsterol and β Sitosterol from Odontonema strictum (Acanthaceae). J. Innov. Pharm. Biol. Sci. 2, 88–96.
[13] Khatun, M., Billah, M., Quader, M. A., 2012. Sterols and sterol glucoside from Phyllanthus species. Dhaka Univ. J. Sci., 60, 5–10.
[14] Manunza, B., Deiana, S., Pintore, M., Gessa, C., 1999. The binding mechanism of urea, hydroxamic acid and N-(N-butyl)-phosphoric triamide to the urease active site. A comparative molecular dynamics study. Soil Biol. Biochem. 31, 789–796.
[15] Stemmler, J. A., Kampf, J. W., Kirk, M. L., Pecoraro, V. L., 1995. A model for the inhibition of urease by hydroxamates. J. Am. Chem. Soc. 117, 6368–6369.
[16] Mouafon, I. L., Mountessou, B. Y. G., Lateef, M., Tchamgoue, J., Shaiq Ali, M., Tchouankeu, J. C., Kouam, S. F. (2023). Atricephenols A and B, two phenolic compounds from Indigofera atriceps Hook. f.(Fabaceae). Natural Product Research, 37(14), 2319-2326.
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    Lidwine, N., Fanny-Aimee, E. M., Caroline, N. N., Julien, N. H., Tsopgni, W. D. T., et al. (2024). Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae). Science Journal of Chemistry, 12(1), 23-26. https://doi.org/10.11648/j.sjc.20241201.13

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    ACS Style

    Lidwine, N.; Fanny-Aimee, E. M.; Caroline, N. N.; Julien, N. H.; Tsopgni, W. D. T., et al. Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae). Sci. J. Chem. 2024, 12(1), 23-26. doi: 10.11648/j.sjc.20241201.13

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    AMA Style

    Lidwine N, Fanny-Aimee EM, Caroline NN, Julien NH, Tsopgni WDT, et al. Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae). Sci J Chem. 2024;12(1):23-26. doi: 10.11648/j.sjc.20241201.13

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  • @article{10.11648/j.sjc.20241201.13,
      author = {Ngah Lidwine and Essombe Malolo Fanny-Aimee and Ngo Nyobe Caroline and Nko’o Henri Julien and Willifred Dongmo Tekapi Tsopgni and Etame Loe Gisele and Jean Duplex Wansi and Kamdem Waffo and Ndom Jean Claude and Mpondo Mpondo Emmanuel},
      title = {Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae)},
      journal = {Science Journal of Chemistry},
      volume = {12},
      number = {1},
      pages = {23-26},
      doi = {10.11648/j.sjc.20241201.13},
      url = {https://doi.org/10.11648/j.sjc.20241201.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20241201.13},
      abstract = {The phytochemical investigation of a previously unstudied species of the genus Apocynaceae, Baissea mortehanii de Wild was undertaken and eight known secondary metabolites were isolated from leaves of this plant including one alkaloid, N-Feruloyltryptamine (1); one aromatic ester, Dibutyl phthalate (2); two flavonoids, Genistein (3) and Gerontoisoflavone A (4), four sterols, β-Sitosterol (5), Sitosterol-3-O-β-D-glucopyranoside (6), Stigmasterol (7) and Stigmasterol-3-O-β-D glucopyranoside (8). The structures of compounds were determined by means of spectroscopic methods :NMR analysis (1H and13C NMR, 1H-1H-COSY, HSQC, HMBC), spectrometric methods such as UV, IR, ESI-MS, EI, and by comparing their data with those reported in the literature. All the isolated compounds were tested for their potential to inhibit the enzyme urease. Urease activity was determined by measuring ammonia production using the indophenol method and thiourea was used as standard inhibitor of urease. Compounds 5 and 7 showed the best urease inhibition with an IC50 value 17. 2 and 18.5 µM respectively, which is higher than that of the potent inhibitor, thiourea (IC50 = 21.5 µM); Compounds 3, 4, 6 and 8 showed a good urease inhibition with an IC50 value 26.9, 29.7, 32.8 and 34.3 µM respectively; Compounds 1 and 2 showed a moderate urease inhibition with an IC50 value 49.1 and 46.8 µM respectively.
    },
     year = {2024}
    }
    

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  • TY  - JOUR
    T1  - Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae)
    AU  - Ngah Lidwine
    AU  - Essombe Malolo Fanny-Aimee
    AU  - Ngo Nyobe Caroline
    AU  - Nko’o Henri Julien
    AU  - Willifred Dongmo Tekapi Tsopgni
    AU  - Etame Loe Gisele
    AU  - Jean Duplex Wansi
    AU  - Kamdem Waffo
    AU  - Ndom Jean Claude
    AU  - Mpondo Mpondo Emmanuel
    Y1  - 2024/02/21
    PY  - 2024
    N1  - https://doi.org/10.11648/j.sjc.20241201.13
    DO  - 10.11648/j.sjc.20241201.13
    T2  - Science Journal of Chemistry
    JF  - Science Journal of Chemistry
    JO  - Science Journal of Chemistry
    SP  - 23
    EP  - 26
    PB  - Science Publishing Group
    SN  - 2330-099X
    UR  - https://doi.org/10.11648/j.sjc.20241201.13
    AB  - The phytochemical investigation of a previously unstudied species of the genus Apocynaceae, Baissea mortehanii de Wild was undertaken and eight known secondary metabolites were isolated from leaves of this plant including one alkaloid, N-Feruloyltryptamine (1); one aromatic ester, Dibutyl phthalate (2); two flavonoids, Genistein (3) and Gerontoisoflavone A (4), four sterols, β-Sitosterol (5), Sitosterol-3-O-β-D-glucopyranoside (6), Stigmasterol (7) and Stigmasterol-3-O-β-D glucopyranoside (8). The structures of compounds were determined by means of spectroscopic methods :NMR analysis (1H and13C NMR, 1H-1H-COSY, HSQC, HMBC), spectrometric methods such as UV, IR, ESI-MS, EI, and by comparing their data with those reported in the literature. All the isolated compounds were tested for their potential to inhibit the enzyme urease. Urease activity was determined by measuring ammonia production using the indophenol method and thiourea was used as standard inhibitor of urease. Compounds 5 and 7 showed the best urease inhibition with an IC50 value 17. 2 and 18.5 µM respectively, which is higher than that of the potent inhibitor, thiourea (IC50 = 21.5 µM); Compounds 3, 4, 6 and 8 showed a good urease inhibition with an IC50 value 26.9, 29.7, 32.8 and 34.3 µM respectively; Compounds 1 and 2 showed a moderate urease inhibition with an IC50 value 49.1 and 46.8 µM respectively.
    
    VL  - 12
    IS  - 1
    ER  - 

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Author Information
  • Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon

  • Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon

  • Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon

  • Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Yaounde, Yaounde, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon

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