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Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh

Received: 11 March 2021     Accepted: 2 April 2021     Published: 23 April 2021
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Abstract

Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC50 (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.

Published in Science Journal of Chemistry (Volume 9, Issue 2)
DOI 10.11648/j.sjc.20210902.13
Page(s) 54-61
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Solvents, Extraction Yield, Antioxidant Activity, Cuminum cyminum, Bangladesh

References
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    Fardushi Rahman, Sabina Easmin, Shahed Zaman, Hamidul Haq, Rezaul Haque Ansary, et al. (2021). Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Science Journal of Chemistry, 9(2), 54-61. https://doi.org/10.11648/j.sjc.20210902.13

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    Fardushi Rahman; Sabina Easmin; Shahed Zaman; Hamidul Haq; Rezaul Haque Ansary, et al. Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Sci. J. Chem. 2021, 9(2), 54-61. doi: 10.11648/j.sjc.20210902.13

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    AMA Style

    Fardushi Rahman, Sabina Easmin, Shahed Zaman, Hamidul Haq, Rezaul Haque Ansary, et al. Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh. Sci J Chem. 2021;9(2):54-61. doi: 10.11648/j.sjc.20210902.13

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  • @article{10.11648/j.sjc.20210902.13,
      author = {Fardushi Rahman and Sabina Easmin and Shahed Zaman and Hamidul Haq and Rezaul Haque Ansary and Subed Chandra Dev Sharma and Suganya Murugesu and Vikneswari Perumal},
      title = {Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh},
      journal = {Science Journal of Chemistry},
      volume = {9},
      number = {2},
      pages = {54-61},
      doi = {10.11648/j.sjc.20210902.13},
      url = {https://doi.org/10.11648/j.sjc.20210902.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20210902.13},
      abstract = {Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC50 (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Antioxidant Screening of Various Solvent Extracts of Cuminum cyminum Cultivated in Bangladesh
    AU  - Fardushi Rahman
    AU  - Sabina Easmin
    AU  - Shahed Zaman
    AU  - Hamidul Haq
    AU  - Rezaul Haque Ansary
    AU  - Subed Chandra Dev Sharma
    AU  - Suganya Murugesu
    AU  - Vikneswari Perumal
    Y1  - 2021/04/23
    PY  - 2021
    N1  - https://doi.org/10.11648/j.sjc.20210902.13
    DO  - 10.11648/j.sjc.20210902.13
    T2  - Science Journal of Chemistry
    JF  - Science Journal of Chemistry
    JO  - Science Journal of Chemistry
    SP  - 54
    EP  - 61
    PB  - Science Publishing Group
    SN  - 2330-099X
    UR  - https://doi.org/10.11648/j.sjc.20210902.13
    AB  - Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC50 (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.
    VL  - 9
    IS  - 2
    ER  - 

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Author Information
  • Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh

  • Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

  • Institute of Tropical Agriculture and Food Security (ITAFoS), University Putra Malaysia, U. P. M. Serdang, Selangor Darul Ehsan, Malaysia

  • Department of Pharmaceutical Technology, Faculty of Pharmacy & Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh, Perak Darul Ridzuan, Malaysia

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